Équipement de fabrication industrielle personnalisée à coût élevé

VC Project Introduction

The manufacture of vitamin C involves four key steps: fermentation, extraction, conversion, and refinement.

1. Fermentation section

The key task is to attach the cleaned strain ramp to the empty, sterile ramp in the strain chamber and then culture to the termination point. after which it was placed in a triangle flask filled with sterile medium and cultivated till maturity. till the very end, cultured in Kirschner flask media. The small fermentation tank's sterile medium was then joined to the seed liquid that had been prepared in a Kirschner flask. After proper culture, the sterile was canned, stored in the refrigerator, and its sterility was verified. The seed was given to fermentation after becoming qualified.

Fermentation starts with a four-stage expansion culture. The expansion of the seed solution occurs during the first, second, and third stages. Fermentation occurs in four stages to produce sorbic sugar. The first step seed solution was connected to the first-level sterile medium for culture to the end point, followed by connections to the second-level sterile medium and the third-level sterile medium for culture to the end point, and finally, connections to fermentation and the fourth-level sterile medium. After the first stage of fermentation in the fermentation tank achieves its conclusion, regulate the temperature, tank pressure, and sterile air flow while the fermentation process is underway, and prepare sugar.

Fermentation in the second step is a four-stage expansion culture. The expansion of the seed solution occurs in stages 1, 2, and 3. By fermenting in four stages, cologne acid sodium is produced. The second step seed solution was connected to the first level of sterile medium for culture to the end point, then to the second level and third level of sterile medium for culture to the end point, and finally to the fourth level of sterile media for fermentation. Temperature, tank pressure, sterile air flow, and the feed liquid's PH were all managed during the fermentation process. Once the fermentation reached its end point, the material was then ready to be discharged.

2. Extraction section

The fermenting liquid is poured into the refinery feeding tank, passed through the oscillating sieve, and then poured into the circulating tank. Dialysate should be pumped into the clear liquid tank after the membrane filtration system has been started for filtering. It is subjected to environmental protection treatment when the concentration produced is kept under 10mg/ml. The dialysate enters the crystallizer to cool and crystalize after desalination by resin, multi-stage concentration. Control the crystallisation temperature to ≤5.0, then use a centrifuge to separate the solid from the liquid to obtain wet gulonic acid. The mother liquid is separated, concentrated twice, cooled to 5–10°C, and then crystallised. Wet cologonic acid that has been isolated from recovered wet cologonic acid enters the conversion step.

3. Conversion section

Quantitative methanol is introduced to the ester conversion tank, followed by a slow addition of concentrated H2SO4 with open stirring. Next, precisely measured wet gulonic acid or recovered gulonic acid is added, and the reaction is heated to completion. The conversion reaction is conducted after the feed material has cooled, and the feed material controls the reaction by being slightly alkaline. After cooling the material down below 10°C for the solid-liquid separation, the reaction is said to have attained its endpoint. Enter the crystallisation tank for chilling after the sodium vitamin C dissolves, desalting and decoloring by ion exchange, and several concentrations. The distillation and purification process is used to prepare the methanol mother liquid for use. The material was separated by centrifugation to produce crude vitamin C after the crystallised liquid was cooled to below 5.0°C by cold brine. Direct refinement is an option, as well as coarsely dissolving the raw vitamin C in clean water before refinement.

By concentrating, crystallising, chilling, and separating the mother liquor of crude vitamin C, the first recovered vitamin C is obtained. This vitamin C is then dissolved into the desalination apparatus for use. The secondary mother liquor was neutralised, the alcohol precipitated, it was chilled, and the sodium cologne acid that had been isolated solidly was refined for desalting. After being separated, the third mother liquor was dealcoholized, decolored, and desalted before being added to the first mother liquor concentration system.

4. Refinery section

Add water to warm up and dissolve crude vitamin C, and carbon to decolorize, in accordance with the ratios specified by the technical specifications. It goes into the crystallisation tank for crystallisation after going through multiple stages of filtration. Use lead crystal technology and precise cooling to manage crystal size distribution. When the feeding of the crystallisation tank is ended, start controlling the chilling speed. When the material has reached the crystallisation stage, add the proper quantity of crystal seed. When the low temperature is reached, add the antifreeze solvent and let it cool to 0 °C or lower before discharging. Utilize a centrifuge machine to separate solids from liquids, and drain the mother liquid's washing liquid. Utilizing a drier, the moist vitamin C is dried. A temperature limit of 65°C or less is set for drying. It takes three hours to dry. The substance is cooled and released upon drying. In order to safeguard the material when drying, the dryer uses a pressure reduction technique. The substance is screened and examined for gold after drying. Following weighing and packaging, storage is necessary once the inspection is successful.

The dealcoholized mother liquor is processed after being desalted in the conversion step. The distillation column corrects the separated crude methanol, which is then employed in the refinery portion.